Second Edition of Biochemistry and Instrumentation Practical Manual
CONTENTS
| Title page Publisher’s page Preface Foreword Contents List of Abbreviations List of Tables List of Figures Practical format Orientation on instruments | i ii iii v vi x xiii xv 1 3 | |
| Experiment no. 1 Experiment no. 2 Experiment no. 3 Experiment no. 4 Experiment no. 5 Experiment no. 6 Experiment no. 7 Experiment no. 8 Experiment no. 9(A) Experiment no. 9(B) Experiment no. 10 Experiment no. 11 Experiment no. 12 Experiment no. 13 Experiment no. 14 Experiment no. 15 Experiment no. 16 Experiment no. 17 Experiment no. 18 Experiment no. 19 Experiment no. 20 Experiment no. 21 Experiment no. 22 Experiment no. 23 Experiment no. 24 Experiment no. 25 Experiment no. 26 Experiment no. 27 Experiment no. 28 Experiment no. 29 Experiment no. 30 Experiment no. 31 Experiment no. 32 Experiment no. 33 Experiment no. 34 Experiment no. 35 Experiment no. 36 Experiment no. 37 Experiment no. 38 Experiment no. 39 Experiment no. 40 Experiment no. 41 Experiment no. 42 Experiment no. 43 Experiment no. 44 Experiment no. 45 Experiment no. 46 Experiment no. 47 Annex I Annex II Annex III Annex IV Annex V Annex VI Annex VII Annex VIII Annex IX Annex X Annex XI Annex XII Annex XIII Annex XIV Bibliography | Basic Techniques Preparation of different types of solutions (Percent, Molar, Normal, Solutions from Solutions and Titrations, Concentrated Solutions Saturated) used in biochemistry laboratory Preparation of different types of Buffers used in biochemical Studies Determination of lmax of different colored and noncolored compounds by spectrophotometer Verification of validity of Beer’s law and determination of the molar extinction coefficient of BSA Qualitative Methods Qualitative detection of proteins and amino acids Qualitative detection of carbohydrates Identification of the nature of carbohydrate in a given sample Isolation and Quantitative Methods Titration curve for the given amino acids Quantitative determination of free amino acid content in serum Comparative study of free amino acid content in germinating and non-germinating seeds Determination of Tyrosine content in a given solution using standard calibration curve Quantitative determination of protein A. Biuret method B. Bradford method C. Folin-Lowry method D. UV method Isolation of Casein from milk Isolation and assay of glycogen from the liver and skeletal muscle Benedict’s test for reducing sugars Determination of soluble carbohydrates by Anthrone Method Determination of acid value of the given fats and oils Determination of the Iodine Number of lipids Determination of saponification value of fats and oils Quantitative analysis of DNA by flourimetry Purification and Identification Methods Preparation of various sub-cellular fractions of liver cells by Differential Centrifugation method Fractionation of serum protein by salting out using Sodium chloride and Ammonium sulphate Precipitation and separation of protein of interest by Trichloroacetic acid method from biological sample Separation and identification of amino acids in a given mixture by Ascending Paper Chromatography Separation and identification of amino acids in a given mixture by Two-dimensional Paper Chromatography Separation and identification of sugars (sugar juices) by adsorption Thin layer chromatography (TLC) Extraction and identification of lipids from given biological samples by Thin Layer Chromatography Purification of proteins by Size Exclusion Chromatography Separation of serum proteins by Ion-exchange Chromatography using cation exchanger Purification of proteins by Affinity Chromatography Analysis of serum protein by Paper Electrophoresis using cellulose acetate paper Determination of molecular weight of a given protein by Sodium Dodecylsulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) Native PAGE (Poly Acrylamide Gel Electrophoresis) of proteins Determination of molecular weight of DNA (genomic and plasmid) by Agarose gel electrophoresis Detection of proteins and DNA in gels by Silver Staining Clinical Biochemistry Determination of blood glucose Quantitative determination of total protein in serum and plasma Determination of Albumin in serum or plasma Determination of Direct and Total Bilirubin in serum Determination of hemoglobin by cyanmethemoglobin method Determination of Serum Glutamic Oxalocetic Transminase (SGOT) activity in serum A. Colorimetric method B. UV kinetic method Determination of Serum Glutamic Pyruvic Transaminase (SGPT) activity in serum A. Colorimetric method B. UV kinetic method Determination of cholesterol in serum A. Enzymatic method (Kit Method) B. Chemical method (ZAK’s method) Quantitative determination of high-density lipoprotein cholesterol Quantitative determinations of triglycerides in serum Quantitative determination of low-density lipoprotein cholesterol Enzymology Enzyme activity of protease and study the effects of temperature, pH, enzyme concentration, time and effectors on the kinetics of protease catalyzed reactions Study of effect of substrate concentration and determination of Vmax and Km value of a-amylase Annexes Buffer Spectroscopy Carbohydrates pKa value Milk Lipids Centrifugation Salting out Chromatography media Buffer systems for chromatography Polyacrylamide gel electrophoresis Molecular weight determination of protein by SDS-PAGE DNA electrophoresis pH indicators | 5 9 12 14 17 19 26 28 31 33 35 38 38 39 41 42 44 47 49 52 54 56 58 60 63 65 68 70 73 76 79 82 85 87 90 92 97 99 101 107 109 111 113 116 119 121 124 126 129 131 133 136 139 142 151 156 160 161 162 163 164 166 168 170 173175 178 179 181 182 |
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