New Book on Practical Microbial Genetics
TABLE OF CONTENTS
Contents | ||
Orientation of Microbial genetics / Molecular Biology Laboratory Theory Micropipettes DNA Extraction Role of Sodium in DNA Extraction Restriction Enzymes Plasmid Bacteriophages Polymerase Chain Reaction-PCR | ||
Experiment no. 1 | Calibration of Micropipettes | |
Experiment no. 2 | Preparation of different Tris buffers: Tris-HCl, Trsi-EDTA and TAE | |
Experiment no. 3 | Isolation of DNA from human blood | |
Experiment no. 4a | Extraction of chromosomal DNA from Gram positive bacteria | |
Experiment no. 4b | Extraction of chromosomal DNA from Gram negative bacteria | |
Experiment no. 5a | Plasmid DNA extraction from bacteria (Alkaline lysis method) | |
Experiment no. 5b | Isolation and purification of bacterial plasmid with Qiagen spin (QIAprep) Miniprep system | |
Experiment no. 6 | RNA extraction from yeast | |
Experiment no. 7 | Spectrophotometric analysis of DNA in extracted solution | |
Experiment no. 8 | Agarose gel electrophoresis of DNA | |
Experiment no. 9a | Demonstration of induction / repression mode of lac-operon regulation system in Escherichia coli | |
Experiment no. 9b | Determination of level of induction of lac-operon in Escherichia coli | |
Experiment no. 10 | Conjugation in bacteria: Co-culture of MDR Salmonella enteritidisTyphi with Escherichia coli HB 101 and detection of transconjugants by antibiotic selection method | |
Experiment no. 11 | Transformation of Escherichia coli HB101 by plasmids: pUC18 or pBR322 | |
Experiment no. 12 | Study of performance of HindIII and EcoRI restriction enzymes | |
Experiment no. 13a | Construction of recombinant DNA: Preparation and purification of insert (Lambda DNA fragments) and vector (pUC18) DNA | |
Experiment no.13b | Construction of recombinant DNA: Ligation of purified insert (Lambda DNA fragments) and vector (pUC18) DNA | |
Experiment no. 14a | Amplification of recombinant DNA: Transformation of recombinant pUC18 DNA in Escherichia coli HB101 | |
Experiment no.14b | Detection of recombinant DNA: Extraction of recombinant pUC18 DNA from transformed Escherichia coli HB101 clones | |
Experiment no. 14c | Detection of recombinant DNA: Extraction of recombinant pUC18 DNA in plasmid extracts of transformed Escherichia coli HB101 clones by agarose gel electrophoresis | |
Experiment no. 15a | Amplification of Escherichia coli DNA (genes) by Polymerase chain Reaction (PCR) | |
Experiment no.15b | Randomly Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) | |
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