A Complete Note on Instrumentation (Analytical Biochemistry)
UPENDRA THAPA SHRESTHA
A Complete Note on Instrumentation (Analytical Biochemistry)
First Edition, November-2016
© Author (Shrestha U.T.)
UPENDRA THAPA SHRESTHA, M.Sc. Microbiology (TU)
Head of Microbiology Department: Kantipur College of Medical Science, Sitapaila, KTM
(B.Sc. and M.Sc. Microbiology affiliated to TU; CMLT and D-Pharmacy affiliated to CTEVT)
Deputy Director: Research Laboratory for Biotechnology and Biochemistry (RLABB)
(Research Scientist)
Faculty: Khwopa College (B.Sc. and M.Sc. Environment Science), Dekocha, Bhaktapur
PGT: Sainik Awasiya Mahavidhyalaya, Sallaghari, Bhaktapur
Email: upendrats@gmail.com
Publisher:
All rights reserved.
No part of this book may be reproduced in any form, by photocopy, microfilm, scanning or any other means, or incorporated into any information retrieval system, electronic or mechanical, without the written permission of the author.
Thank you!
UNIT-I
· 1.1 Physiological Solutions
· 1.2 Cell Disruption
o Mechanical Cell Disruption
o Non-mechanical Cell Disruption
§ Physical Methods
§ Chemical Methods
§ Biological Methods
UNIT-II
· Protein Purification
o Background knowledge about protein
o Protein Isolation/Precipitation
o Protein Purification
o Protein Determination
o Protein Assay
UNIT-III
· Centrifugation
o Principle of Centrifugation
o Centrifuges and its types
o Differential Centrifugation
o Density Gradient Centrifugation
UNIT-IV
· Chromatography
o Paper Chromatography
o Thin Layer Chromatography
o Column Chromatography
§ Size Exclusion Chromatography
§ Ion Exchange Chromatography
§ Affinity Chromatography
§ Gas Chromatography
§ High Performance Liquid Chromatography (HPLC)
UNIT-V
· Electrophoresis
o Principle of Electrophoresis
o Media used in Electrophoresis
o Agarose Gel Electrophoresis
o Isoelectric Focus Gel Electrophoresis
o SDS-PAGE
o 2D Gel Electrophoresis
o Western Blotting
UNIT-VI
· Spectroscopy
o UV-Visible Spectrophotometry
§ Colorimeter/Photometer/Spectrophotometer
o Spectroflouremetry
o Molecular Luminescence Spectroscopy
o Atomic Spectroscopy
§ Atomic Absorption Spectroscopy
§ Atomic Emission Spectroscopy
§ Atomic Florescent Spectroscopy
o Mass Spectroscopy
o Nuclear Magnetic Resonance
UNIT-VII
· Microscopy
o Electron Microscopy
§ Transmission Electron Microscopy
§ Scanning Electron Microscopy
o Phase Contrast Microscopy
o Fluorescent Microscopy
o Gel-Doc System
UNIT-VIII
· Polymerase Chain Reaction
· Microarrays
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