CDM, TU successfully conducted training on "Basic Molecular Biology Techniques

Central Department of Microbiology, TU has successfully conducted five days "Training on Basic Molecular Biology" for the faculties from B.Sc. Microbiology running colleges all over the country on Jestha 7-11, 2075 supported by University Grants Commission (UGC), Sanothimi, Bhaktapur, Nepal. The program was inaugurated by Prof. Dr. Ram Pd. Khatiwada, Dean, Institute of Science and Technology (IoST), TU. A total of 20 faculties have been participated on the training from B.Sc. Microbiology running colleges all over the country.
Photograph 1: Trainees doing practical works during training

Photograph 2: Inauguration program of training on Basic Molecular Biology Techniques 

Photograph 3: Prof. Dr. Ram P. Khatiwada, dean; IoST giving his remarks on the training program
Photograph 4: Group photo session of experts and trainees after the training

Training on Basic Molecular Biology techniques

Agenda of the workshop

Venue: Central Department of Microbiology, Tribhuvan University
Date: 2075/2/7-11 (21-25 May 2018)
Time
Particulars
Responsible persons
DAY I: 2075/2/7 (May 21, 2018) Monday
10:30-11:00
Registration
11:00-11:30
Inaugural session

Chair of the programme
Head, Central Department of Microbiology, T.U.
Introduction of the participants

Welcome speech and remarks
Prof. Dr. Anjana Singh
Remarks
Dr. Prakash Ghimire
Remarks
UGC representative
Opening of the programme and remarks
Prof. Dr. Ram Prasad Khatiwada, Dean, Institute of Science and Technology, T.U.
Closing of inaugural session
Head, Central Department of Microbiology, T.U.
11:30-11:45
Tea break

11:45-12:00
Overview of the training program
Dr. Megha Raj Banjara
12:00-1:00
Reagents preparation
Prof. Dr. Anjana Singh
1:00-2:00
LUNCH BREAK
2:00-5:00
Reagents preparation
1.   Shaila Basnyat
2.   Upendra Thapa Shrestha

DAY II: 2075/2/8 (May 22, 2018) Tuesday
10:30-11:30
DNA/Plasmid extraction method-theory
Dr. Prakash Ghimire
11:30-1:00
Practical session I-DNA extraction from Gram+ve and Gram-ve bacteria
1.      Dr. Dev Raj Joshi
2.      Upendra Thapa Shrestha
1:00-2:00
LUNCH BREAK
2:00-5:00
Practical session II-Plasmid extraction from Gram-ve bacteria
1.      Dr. Megha Raj Banjara
2.      Nabaraj Adhikari

Day III: 2075/2/9 (May 23, 2018) Wednesday
10:30-11:00
DNA and RNA extraction by kit method-theory
Assoc. Prof. Binod Lekhak
11:00-11:45
Spectrophotometry and electrophoresis-theory
Dr. Dev Raj Joshi
11:45-1:30
Practical session I-DNA and RNA extraction by kit method
1.   Assoc. Prof. Binod Lekhak
2.   Reshma Tuladhar
1:30-2:00
LUNCH BREAK
2:00-5:00
Practical session II- Spectrophotometry and electrophoresis
1.    Prof. Dr. Anjana Singh
2.    Dr. Dev Raj Joshi

Day IV:  2075/2/10 (May24, 2018) Thursday
10:30-11:30
PCR technique-theory
Dr. Megha Raj Banjara
11:30-1:00
Practical session I-PCR reaction mixture preparation
1.    Reshma Tuladhar
2.    Supriya Sharma
1:00-2:00
LUNCH BREAK
2:00-5:00
Practical session II- PCR running
1.    Assoc. Prof. Binod Lekhak
2.    Nabaraj Adhikari

Day V:  2075/2/11 (May25, 2018) Friday
10:30-11:30
Post PCR analysis-theory
Ms. Reshma Tuladhar
11:30-1:00
Practical session I-Post PCR analysis
1.    Dr. Prakash Ghimire
2.    Upendra Thapa Shrestha
1:00-2:00
LUNCH BREAK
2:00-3:00
Closing and certificate distribution

Master of ceremony: Mr. Upendra Thapa Shrestha

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