Central Department of Microbiology, TU has successfully conducted five days "Training on Basic Molecular Biology" for the faculties from B.Sc. Microbiology running colleges all over the country on Jestha 7-11, 2075 supported by University Grants Commission (UGC), Sanothimi, Bhaktapur, Nepal. The program was inaugurated by Prof. Dr. Ram Pd. Khatiwada, Dean, Institute of Science and Technology (IoST), TU. A total of 20 faculties have been participated on the training from B.Sc. Microbiology running colleges all over the country.
Photograph 1: Trainees doing practical works during training
Photograph 2: Inauguration program of training on Basic Molecular Biology Techniques
Photograph 3: Prof. Dr. Ram P. Khatiwada, dean; IoST giving his remarks on the training program
Photograph 4: Group photo session of experts and trainees after the training
Training on Basic Molecular Biology techniques
Agenda of the workshop
Venue: Central Department of Microbiology, Tribhuvan University
Date: 2075/2/7-11 (21-25 May 2018)
| Time | Particulars | Responsible persons |
| DAY I: 2075/2/7 (May 21, 2018) Monday | ||
| 10:30-11:00 | Registration | |
| 11:00-11:30 | Inaugural session | |
| Chair of the programme | Head, Central Department of Microbiology, T.U. | |
| Introduction of the participants | | |
| Welcome speech and remarks | Prof. Dr. Anjana Singh | |
| Remarks | Dr. Prakash Ghimire | |
| Remarks | UGC representative | |
| Opening of the programme and remarks | Prof. Dr. Ram Prasad Khatiwada, Dean, Institute of Science and Technology, T.U. | |
| Closing of inaugural session | Head, Central Department of Microbiology, T.U. | |
| 11:30-11:45 | Tea break | |
| 11:45-12:00 | Overview of the training program | Dr. Megha Raj Banjara |
| 12:00-1:00 | Reagents preparation | Prof. Dr. Anjana Singh |
| 1:00-2:00 | LUNCH BREAK | |
| 2:00-5:00 | Reagents preparation | 1. Shaila Basnyat 2. Upendra Thapa Shrestha |
| | ||
| DAY II: 2075/2/8 (May 22, 2018) Tuesday | ||
| 10:30-11:30 | DNA/Plasmid extraction method-theory | Dr. Prakash Ghimire |
| 11:30-1:00 | Practical session I-DNA extraction from Gram+ve and Gram-ve bacteria | 1. Dr. Dev Raj Joshi 2. Upendra Thapa Shrestha |
| 1:00-2:00 | LUNCH BREAK | |
| 2:00-5:00 | Practical session II-Plasmid extraction from Gram-ve bacteria | 1. Dr. Megha Raj Banjara 2. Nabaraj Adhikari |
| | ||
| Day III: 2075/2/9 (May 23, 2018) Wednesday | ||
| 10:30-11:00 | DNA and RNA extraction by kit method-theory | Assoc. Prof. Binod Lekhak |
| 11:00-11:45 | Spectrophotometry and electrophoresis-theory | Dr. Dev Raj Joshi |
| 11:45-1:30 | Practical session I-DNA and RNA extraction by kit method | 1. Assoc. Prof. Binod Lekhak 2. Reshma Tuladhar |
| 1:30-2:00 | LUNCH BREAK | |
| 2:00-5:00 | Practical session II- Spectrophotometry and electrophoresis | 1. Prof. Dr. Anjana Singh 2. Dr. Dev Raj Joshi |
| | ||
| Day IV: 2075/2/10 (May24, 2018) Thursday | ||
| 10:30-11:30 | PCR technique-theory | Dr. Megha Raj Banjara |
| 11:30-1:00 | Practical session I-PCR reaction mixture preparation | 1. Reshma Tuladhar 2. Supriya Sharma |
| 1:00-2:00 | LUNCH BREAK | |
| 2:00-5:00 | Practical session II- PCR running | 1. Assoc. Prof. Binod Lekhak 2. Nabaraj Adhikari |
| | ||
| Day V: 2075/2/11 (May25, 2018) Friday | ||
| 10:30-11:30 | Post PCR analysis-theory | Ms. Reshma Tuladhar |
| 11:30-1:00 | Practical session I-Post PCR analysis | 1. Dr. Prakash Ghimire 2. Upendra Thapa Shrestha |
| 1:00-2:00 | LUNCH BREAK | |
| 2:00-3:00 | Closing and certificate distribution | |
Master of ceremony: Mr. Upendra Thapa Shrestha




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